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Congresso Brasileiro de Microbiologia 2023
 		Resumo: 348-1

348-1

CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY OF ESSENTIAL OIL FROM Croton urticifolius Lam. AGAINST Sthaphylococcus aureus STRAINS

Autores:
Ellen Araújo Malveira (UFC - Universidade Federal do Ceará) ; Aluska Kelly Alves Nunes (UERN - Universidade do Estado do Rio Grande do Norte) ; Alexandre Lopes Andrade (UFC - Universidade Federal do Ceará) ; Edson Holanda Teixeira (UFC - Universidade Federal do Ceará) ; Mayron Alves de Vasconcelos (UEMG - Universidade do Estado de Minas Gerais)

Resumo:
Biofilms are microbial communities resistant embedded in an extracellular polymeric matrix. Biofilms may be developed by several resistant microorganisms associated to hospital-acquired infections, included Staphylococcus aureus. Natural products, such as essential oils, can present antimicrobial and antibiofilm activity against resistant strains. Thus, the aim of this work was to evaluate the chemical composition of the essential oil extracted from Croton urticifolius Lam. leaves and to evaluate its antimicrobial and antibiofilm effects against S. aureus ATCC 700698 and ATCC 25923. The plant material was collected in Serra do Lima, municipality of Patu-RN. The essential oil was extracted from the leaves by hydrodistillation method and the chemical composition was identified by Gas chromatography-mass spectrometry (GC-MS). Thirty-two compounds were identified by GC-MS. The majority compounds were benzene1,2,3-trimethoxy-5-(2-propenyl (39.72%); eucalyptol (19.03%), caryophyllene (5.36%); 3,5 - dimethoxytoluene (4.88%); methyleugenol (4.12%). The antimicrobial activity of the oil was evaluated by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays. The antibiofilm analyses were performed by quantification of biomass through crystal violet (CV) staining and number of viable cells counting. The essential oil showed MIC and MBC values at concentration of 2.5% against S. aureus ATCC 700698 (Methicillin-resistant Staphylococcus aureus - MRSA), and MIC at 2.5% against S. aureus ATCC 25923. However, the oil did not show MBC against S. aureus ATCC 25923. Regarding biomass quantification, was observed significantly reduction in the S. aureus ATCC 700698 and ATCC 25923 biofilms in all concentrations tested (5 to 0.078%). Moreover, a reduction of 2 to 5 log in the number of viable cells on S. aureus ATCC 700698 biofilms was observed at concentrations of 5 to 1.25% of oil and a 1 log reduction at concentrations of 5 and 2.5% against S. aureus ATCC 25923. In conclusion, the results show that the C. urticifolius essential oil are a promising bioactive source from compounds against infections caused by S. aureus and its biofilm.

Palavras-chave:
 antimicrobial resistance, biofilms, Croton urticifolius, essential oil, Staphylococcus aureus


Agência de fomento:
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG).